The Grand Ole Opry will celebrate the 25th anniversary of the landmark O Brother, Where Art Thou? soundtrack with a special live show on Saturday, February 28, bringing together many of the artists who helped make the album a cultural phenomenon.

The evening will feature performances by Alison Krauss, Emmylou Harris, Dan Tyminski, Del McCoury, Billy Strings, Old Crow Medicine Show, Molly Tuttle, The Fairfield Four, The Whites and Tim Blake Nelson, along with Alaskan Sunnyside Sisters, Chris Thomas King, Colin Linden, Fisk Jubilee Singers, Jerry Douglas and Sarah Jarosz.

Released in 2000, the soundtrack to the Coen Brothers film was produced by T Bone Burnett and became one of the most influential roots music collections of the modern era. Blending bluegrass, gospel, blues, country and folk, the album went on to sell more than eight million copies and reached No. 1 on the Billboard 200 chart.

The album earned multiple Grammy Awards, including Album of the Year, and was also named Album of the Year by the Country Music Association, the Academy of Country Music and the International Bluegrass Music Association. Billboard later named it the biggest selling soundtrack of the decade.

The film starred George Clooney, John Turturro and Tim Blake Nelson as three escaped convicts traveling through Depression era Mississippi, and its music helped introduce a new generation of listeners to traditional American roots sounds. Critics have widely credited the soundtrack with sparking a modern folk and Americana revival.

In addition to the live performances, fans attending the Opry show will have the chance to purchase an exclusive 25th anniversary O Brother, Where Art Thou? Hatch Show Print poster, available in The Opry Shop.

Lost Highway Records will also mark the milestone with the release of a 25th anniversary vinyl gatefold edition of the soundtrack on February 20, 2026.

Founded in 1925, the Grand Ole Opry has long served as a showcase for traditional and contemporary country music, and organizers say the anniversary event reflects the same spirit of honoring musical heritage while continuing to evolve.

Tickets and additional information about the February 28 celebration are available through the Grand Ole Opry.

Author: Andrew Braithwaite

Andrew is the founder and chief editor of Music Talkers. He's also a keen music enthusiast and plays the guitar.